Quantitation of the tetramethyl-p-phenylenediamine oxidase reaction in Neisseria species.
نویسندگان
چکیده
The tetramethyl-p-phenylenediamine oxidase reaction commonly used in the Kovacs oxidase test was quantitatively estimated for various Neisseria species employing standardized resting cell suspensions. This genus of microorganisms exhibited very high tetramethyl-p-phenylenediamine oxidase rates comparable to that of Azotobacter and Pseudomonas, and this reaction was found to be a valid measurement for the respiratory capability possessed by this group of organisms.
منابع مشابه
Reduction and activity of cytochrome c in the cytochrome c-cytochrome aa3 complex.
Uncharged reductants, such as NNN'N'-tetramethyl-p-phenylenediamine and diaminodurene, reduce cytochrome c at both high and low ionic strength, unlike ascorbate, which is effective only at low ionic strength. The 'tightly bound' cytochrome c-cytochrome c oxidase complex, with 1 equiv. of cytochrome c per cytochrome aa3, can be prepared by simple mixing of the two component species. Its properti...
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ورودعنوان ژورنال:
- Applied microbiology
دوره 28 6 شماره
صفحات -
تاریخ انتشار 1974